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Anti-Nuclear Antibodies (ANA)
Anti-Nuclear Antibodies (ANA)
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Anti-Nuclear Antibodies (ANA)
Category: Immune response.
Subcategory: Auto-antibody tests.
Material studied: Blood.
Estimated cost of test: $16.00.
Patient time for test: 5-10 minutes.
Reliability of test results: Not specific for any disease, but screens for several autoimmune* disorders.
Available as home self-test? No.
Note: Test evaluates the immune system by measuring anti-nuclear antibodies* in blood. Further testing is required for accurate diagnosis.
BEFORE THE TEST
Purpose of test:
Screens for systemic lupus erythematosus*.
Monitors effectiveness of treatment with drugs for systemic lupus erythematosus*.
Where is test performed?
Commercial laboratory, hospital, doctor's office.
Who performs test?
Lab technician, nurse, doctor.
Risks and precautions:
If tourniquet is applied on the arm too long (over 1 minute), it may cause an inaccurate test result. Request another sample to be collected to ensure accuracy.
Patient preparation:
Activity--No changes necessary.
Diet--No changes necessary.
Medicines--Inform the person performing the test if you have recently taken any medications listed under Taking these drugs may affect test results. You may be asked not to take this medication before the test.
Disrobing--None required. Roll up sleeve only.
THE TEST
Sensory factors:
Touching--You will feel mild discomfort when the needle is inserted into the vein or when the lancet pricks a finger, heel or ear lobe.
Seeing--You will see the technician, nurse or doctor, the basket or tray to hold the equipment, the needles, syringes, collecting tubes and bandages.
Feeling--Some degree of apprehension or fear is normal and should be expected. Discomfort disappears when the test is finished.
Other senses (taste, smell, hearing)--Not affected.
Equipment used:
Needles, syringes and heparinized* collecting tubes. Sterile, disposable equipment prevents contamination or spread of infection. There is no risk of becoming infected with the hepatitis virus, AIDS virus or any other infecting germ.
Description of test:
Technician, doctor or nurse applies a tourniquet or blood-pressure cuff to the upper arm if blood is collected from a vein.
Skin over the vein to be stuck is cleaned with alcohol or other antiseptic on a piece of cotton.
When blood is drawn from a vein, the operator feels the vein to be used then punctures both the skin and vein in one quick stroke. The needle used is a sterile, disposable needle attached to a sterile, disposable syringe.
Operator withdraws the needle and transfers sample from the collecting syringe into sterile tubes (identified with your name) before sending samples to the laboratory for analysis. Tubes are treated with an anti-coagulant chemical to prevent clotting.
If blood is collected from a finger, heel or ear lobe, skin over the selected site is cleaned with an antiseptic. The operator quickly pierces the skin to a shallow depth, using a sterile, disposable metal lancet. The drop or two of blood produced is collected into a capillary pipette.
AFTER THE TEST
Immediate post-test care:
Apply pressure to the puncture site with cotton provided by the laboratory.
If a vein has been punctured, raise your entire arm over your head while applying pressure.
Some discoloration, soreness or swelling may develop at the venepuncture site. This responds well to moist, warm compresses applied every 2 to 4 hours.
Activity after test:
Keep a clean, dry bandage on the site where blood was drawn for 24 hours.
Be alert for any signs of infection at the site where blood was drawn. Report any changes to your doctor immediately.
Resume taking any medications that were withheld before the test.
Return to pretest activities right away.
Use warm compresses if blood collects under the puncture site or if the area becomes tender, red or painful.
Time before test results available:
4 to 5 days.
TEST RESULTS
Test values:
Test results are determined by immunofluorescence* and ultraviolet microscopy*.
Normal values: Test for ANA is negative at a titer* of 1:32 or below.
What absence of ANA may indicate:
Rules out systemic lupus erythematosus*.
What low titers* may indicate:
Viral diseases.
Liver disease.
Collagen-vascular disease*.
Autoimmune* disease.
Taking these drugs may affect test results:
Acetazolamide.
Aminosalicylic acid (PAS).
Chlorothiazide.
Chlorpromazine.
Chlorprothixene.
Clofibrate.
Ethosuximide.
Gold salts.
Griseofulvin.
Hydralazine.
Isoniazid.
Mephenytoin.
Methyldopa.
Methysergide.
Oral contraceptives.
Para-aminosalicylic acid.
Penicillin.
Phenylbutazone.
Phenytoin.
Primidone.
Procainamide.
Propylthiouracil.
Quinidine.
Reserpine.
Streptomycin.
Sulfonamides.
Tetracyclines.
Thiouracil.
Trimethadione.
Other factors that may affect test results: None expected.
*See Glossary.
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